Elektronik ve Haberleşme Mühendisliği Bölümü Yayın Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12416/260
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Article Citation - WoS: 48Citation - Scopus: 50High-Throughput Screening of Large Volumes of Whole Blood Using Structured Illumination and Fluorescent On-Chip Imaging(Royal Soc Chemistry, 2012) Arpali, Serap Altay; Arpali, Caglar; Coskun, Ahmet F.; Chiang, Hsin-Hao; Ozcan, AydoganUndiluted blood samples are difficult to image in large volumes since blood constitutes a highly absorbing and scattering medium. As a result of this limitation, optical imaging of rare cells (e.g., circulating tumour cells) within unprocessed whole blood remains a challenge, demanding the use of special microfluidic technologies. Here we demonstrate a new fluorescent on-chip imaging modality that can rapidly screen large volumes of absorbing and scattering media, such as undiluted whole blood samples, for detection of fluorescent micro-objects at low concentrations (for example <= 50-100 particles/mL). In this high-throughput imaging modality, a large area microfluidic device (e.g., 7-18 cm(2)), which contains for example similar to 0.3-0.7 mL of undiluted whole blood sample, is directly positioned onto a wide-field opto-electronic sensor-array such that the fluorescent emission within the microchannel can be detected without the use of any imaging lenses. This microfluidic device is then illuminated and laterally scanned with an array of Gaussian excitation spots, which is generated through a spatial light modulator. For each scanning position of this excitation array, a lensfree fluorescent image of the blood sample is captured using the opto-electronic sensor-array, resulting in a sequence of images (e.g., 144 lensfree frames captured in similar to 36 s) for the same sample chip. Digitally merging these lensfree fluorescent images based on a maximum intensity projection (MIP) algorithm enabled us to significantly boost the signal-to-noise ratio (SNR) and contrast of the fluorescent micro-objects within whole blood, which normally remain undetected (i.e., hidden) using conventional uniform excitation schemes, involving plane wave illumination. This high-throughput on-chip imaging platform based on structured excitation could be useful for rare cell research by enabling rapid screening of large volume microfluidic devices that process whole blood and other optically dense media.Article Citation - WoS: 8Citation - Scopus: 12Implementation and Characterization of an Absorption Filter for On-Chip Fluorescent Imaging(Elsevier Science Sa, 2017) Arpali, Caglar; Arpali, Serap Altay; Yildirim, EnderHere we present fabrication and characterization of an absorption filter with superior roll-on properties and precisely tunable cut-off wavelengths for fluorescent imaging applications in lab-on-a-chip systems. The filters were fabricated by spinning dye doped photopolymer (Orasol Yellow in Norland Optical Adhesive 60) on glass substrates. The fabrication technique allowed us to precisely tune the cut-off wavelength of the filters. We showed that filters with different cut-off in the range of 386 nm-504 nm could be obtained simply by controlling the settling time before spinning. The filters exhibited a steep roll-on from stopband to passband at the cut-off. Transmission in the stopband was observed to be maximum 3% while it was almost constant at 100% in the passband within the range of 220 nm-620 nm. On-chip use of the filters was also demonstrated for imaging particular fluorescent beads. (C) 2016 Elsevier B.V. All rights reserved.