Makine Mühendisliği Bölümü Yayın Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12416/263

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Author: search.filters.author.Yildirim, E.Publisher: search.filters.publisher.Chemical and Biological Microsystems Society

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  • Conference Object
    Citation - Scopus: 1
    A Droplet Based Multi-Drug Screening System Controlled With Electrostatic Microvalves
    (Chemical and Biological Microsystems Society, 2012) Yildirim, E.; Yıldırım, Ender; Özgür, E.; Külah, H.; Makine Mühendisliği
    This paper presents a droplet-based drug effect analysis system utilizing electrostatically-actuated normallyclosed microvalves to screen the effect of multiple drugs on a single type of cell. Proposed system minimizes the need for off-chip equipment by utilizing parylene based electrostatic microvalves. Prototypes of the system were fabricated and tested using colored DI water and 3 μm diameter micro beads, emulating drugs and cells respectively. During the tests, micro beads could be successfully entrapped in 137 pl droplets. Tests carried out with yeast cells also yielded successful encapsulation of the cells. It was shown that, switching between the drugs could be achieved by applying 200 V dc to operate the microvalves.
  • Conference Object
    Phaseguide Assisted Liquid Lamination for Magnetic Bead-Based Assays
    (Chemical and Biological Microsystems Society, 2013) Phurimsak, C.; Yıldırım, Ender; Yildirim, E.; Trietsch, S.J.; Hankemeier, T.; Tarn, M.D.; Pamme, N.; Vulto, P.; Makine Mühendisliği
    We demonstrate a simple, pump-free platform for performing rapid magnetic bead-based processes via their transfer through sequentially laminated liquid streams, made possible by the use of phaseguide technology. We have applied this strategy to two on-chip assays: (i) a streptavidin-biotin binding assay, and (ii) a sandwich immunoassay for the detection of C-reactive protein (CRP). Here, functionalized magnetic beads were pulled through alternating lanes of reagents and buffer solution, allowing multiple binding and washing processes to be reduced into a single step, significantly shortening procedural times compared to conventional multi-step bead-based assays.