Experimental Design and Optimization for the Spectral Analysis of Two-Component Mixture

Abstract

A chemometric experimental design was applied to obtain the optimal settings for spectrophotomeric quantitative resolution of two-component mixture containing benazepril hydrochloride (BE) and hydrochlorothiazide (HCT) in tablets. As it is known, the digital parameters for instance divisor concentration and All-interval are very important concepts for the application of the ratio spectra derivative spectrophotometry to the resolution of binary mixture systems. In this manuscript, a three-level full factor design was used to optimize the effects of the divisor concentration and Delta lambda-interval on the quantitative analysis of the above mentioned drugs in samples. Desirable results were obtained: firstly, with the divisor concentrations and Delta lambda-interval, 21.20 mu g/mL and Delta lambda=27.50 nm for BE in the ratio spectra first derivative spectrophotometry, secondly 27.20 mu g/mL and Delta = 5.4 for HCT in the ratio spectra second derivative method Under the above mentioned conditions, both first and second derivative approaches showed good precision, accuracy and linearity in the quantitative analysis of BE and HCT in two different pharmaceutical tablet products.